This method could be easily applied to other transgenic mice. Our results demonstrate that SSCP is a simple, accurate, repeatable and efficient method for the routine genotyping of this current AD model. Here we show that this small mutated DNA sequence can affect its secondary structure resulting in altered mobility that can be easily detected on a polyacrylamide gel, by the single-strand conformation polymorphism (SSCP) technique. In PS1M146VKI mice, only three nucleotides are modified in the gene. Genotyping PS1M146VKI line requires many steps and thus a large quantity of DNA. PS1M146VKI mice that express the PS1M146V targeted allele at normal physiological levels and triple-transgenic model (3×Tg-AD) derived from homozygous PS1M146VKI mice were generated to study the pathogenesis of AD. Vianna1,2, Daly Noll1, Isidora Mura-Jornet1, Paulina Valenzuela-Guerra1, Daniel González-Acuña3, Cristell Navarro4, David E. Mutations in the presenilin-1 (PS1) gene are causally linked to many cases of early-onset inherited Alzheimer's disease (AD). Comparative genome-wide polymorphic microsatellite markers in Antarctic penguins through next generation sequencing Juliana A. In this study, we demonstrate a powerful alternative for genotyping using presenilin-1 mutant knock-in (PS1M146KI) mice as an example. In addition, the multiplication of transgenes included in mouse models of human disease underlines the importance of correct controls and the fact that investigators need an accurate and rapid genotyping of the littermates generated. The use of transgenic mice expressing point mutations demands that the detection of the different alleles is efficient and reliable.
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